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The electrochemical detection of sensitively engineered supramolecular systems with nanostructures has long been sought after. Although the principles of sensing have been described, few strategies are available for their implementation. A new sensing strategy, “turn-on” detection using electroactive dendritic polymers, has been developed. The sensing features are: (1) electrochemical detection of peptide self-assembly using electroactive dendritic polymeric materials (1), (2) fast and efficient molecular recognition of peptides through electroactive dendritic polymeric recognition units (2) and (3) improved sensing sensitivity and selectivity by the incorporation of electroactive dendritic polymeric materials (3). The application of the sensing strategy was demonstrated in sensing the solution-phase self-assembly of histidine-rich peptides. The electroactive dendritic polymeric materials are proven to be excellent tools for sensing peptide self-assembly based on their capability of (1) good electrochemical response from complexes through electron and energy