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and environmental safety, and (4) assess the quality of the. Social Services Workforce in Occupational Safety and Health David vinson’s particles vol 1 for particular Salil Kranti John, Intense focus on everything related to work safety.The effect of pharmaceutical grade methylmercury on chromatin condensation in mouse spermatocytes.
Male mice are more prone than females to developing neurological disorders after mercury (Hg) exposure. Although spermatogenesis and sperm maturation are sensitive to Hg-induced disorders, the mechanism of Hg toxicity is not fully understood. Additionally, it has been suggested that Hg influences the chromatin structure of germ cells, although evidence for this hypothesis is limited. Our study investigated the effect of pharmaceutical-grade methylmercury on the chromatin structure of mouse spermatocytes. We observed a significant increase in the number of protamines per unit of DNA in spermatocytes cultured with methylmercury. The number of DNA-histone complexes in isolated nuclei from mice treated with methylmercury (2000 mg kg(-1) week(-1) for 10 weeks) was not significantly different compared to the controls. We conclude that pharmaceutical-grade methylmercury does not have a major effect on the chromatin of mouse spermatocytes.Alteration of cell cycle-related genes in fulminant hepatic failure.
We aimed to determine the expression levels of cell cycle-related genes in fulminant hepatic failure (FHF). We analyzed the expression levels of Ki-67, p16(INK4A), cyclin D1, cyclin D3, and cyclin-dependent kinase (Cdk) 2A in the cytosol by using real-time polymerase chain reaction. The protein levels of cyclin D1 and p16(INK4A) were determined by Western blot. The Ki-67 protein level was evaluated by immunohistochemistry. The p16(INK4A) mRNA levels in fulminant hepatic failure (FHF) were significantly lower than those in healthy controls. The Ki-67 protein levels and Ki-67 mRNA levels were increased in FHF. The cyclin D1 protein levels were significantly increased,

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